Glyphosate: Herbicide ANP Retention of an Extremely Polar Compound

Column: Cogent Diamond HydrideTM 4μm, 100Å. Catalog No.: 70000-15P-2 Dimensions: 2.1 x150 mm Solvents: A: DI water + 5 mM ammonium acetate B: 90% acetonitrile/10% DI water/10 mM ammonium acetate Gradient: 0.0 min – 80.0% B 5.0 min 5%B 1 .0 min 80%B 6.0 min to 80%B 1.1 min to 5%B Post Tme: 5 min Flow Rate: 0.5 mL/min. Sample Prep: Glyphosate: 168.0067m/z (M H)Figure A: injection #1, RT = 3.365 min Figure B: injection #5, RT = 3.366 min Sample stock solution was purchased from Sigma (1000 mcg/mL). Sample for analysis was made by diluting the stock 1:100 in 30:70 solution A and B. Detection: ESI – neg Agilent 6210 MSD TOF mass spectrometer.